DPP4 Activity Fluorometric Assay Kit

Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. The substrates of CD26/DPP4 are proline (or alanine) containing peptides and include growth factors, chemokines, neuropeptides, and vasoactive peptides. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1 and hence its inhibition by drugs such as Sitagliptin have been used for treatment of diabetes mellitus type 2. DPP4 also appears to work as a suppressor in the development of cancer and tumors. In BioVision’s DPP4 Activity Assay Kit, DPP4 cleaves a substrate to release a quenched fluorescent group, AMC (7-Amino-4-Methyl Coumarin), (Ex/Em = 360/460 nm). This assay is rapid, simple, sensitive, and reliable, as well as, suitable for high throughput activity screening of DPP4. This kit detects DPP4 activity as low as 3 µU per well.

• Detection method: Fluorescence (Ex/Em 360/460 nm) • Species reactivity: Mammalian • Applications: This kit detects DPP4 activity as low as 3 µU per well.

DPP4

none

• DPP4 Assay Buffer • DPP4 Substrate (H-Gly-Pro-AMC) • DPP4 Positive Control • AMC Standard (10 μM) • DPP4 Inhibitor (Sitagliptin)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture lysates, plasma, serum, other biological fluids, cell culture medium, etc.

Biovision supplies other types of Assays as 1.