Protease Activity Fluorometric Assay Kit

Proteases are naturally present in all organisms. These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to highly regulated cascades. Proteases can either break specific peptide bonds (limited proteolysis), depending on the amino acid sequence of a protein, or break down a complete peptide to amino acids (unlimited proteolysis). The activity can be a destructive change (abolishing a protein's function), an activation of a function (preform to mature form) or it can be a signal in a signaling pathway. BioVision’s Protease Activity Assay Kit is designed for the quantitative determination of proteases present in the protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-Casein is highly quenched. Upon digestion by proteases present in the sample the FITC-Casein substrate is cleaved into smaller peptides which abolishes the quenching of the fluorescence label. The fluorescence of the FITC-labeled peptide fragments is measured at Ex/Em = 485/530 nm. The kit is supplied with our Mass Spectrometry Grade (MSG), chemically stabilized Trypsin for use as a general protease control. However, other protease standard controls can also be used. This kit is easy to use and can detect < 500 pg/well Trypsin present in the sample.

• Detection method: Fluorescence (Ex/Em 485/530 nm) • Applications: This kit can detect < 500 pg/well Trypsin present in the sample.

• Simple procedure; takes ~60 minutes • Fast and convenient • Sensitive assay for measuring protease activity in a number of sample types

Protease

none

• Assay Buffer • Substrate (lyophilized) • FITC Standard (25 μM) • Positive Control (lyophilized)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food samples, etc.

Biovision supplies other types of Assays as 1.