Glucose Dehydrogenase Activity Colorimetric Assay Kit

Catalog number
K786-100
Name
Glucose Dehydrogenase Activity Colorimetric Assay Kit
Size
100 assays
Price
429.00 EUR
Supplier

Details

Kit's description
Glucose 1-dehydrogenase (NADᶧ) (EC 1.1.1.118 ) is an enzyme that catalyzes the chemical reaction: D-glucose + NADᶧ↔D-glucono-1,5-lactone + NADH + H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NADᶧ or NADPᶧ as acceptor. BioVision’s Glucose Dehydrogenase (GDH) Assay Kit provides a convenient tool for sensitive detection of the GDH in a variety of samples. The GDH present in sample will recognize D-glucose as a specific substrate leading to a proportional color development. The activity of GDH can be easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01 mU with our unit definition.
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: This assay detects GDH activity as low as 0.01 mU with our unit definition.
Kit's benefits
• Simple procedure; takes ~ 30 minutes to 2 hours • Fast and convenient • Sensitive assays for measuring Glucose Dehydrogenase in various biological samples
Category
Glucose Dehydrogenase
Kit's other name
none
Contents
• GDH Assay Buffer • Glucose (2 M) • Developer (Lyophilized) • GDH Positive Control (Lyophilized) • NADH Standard (0.5 μmol, Lyophilized)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples, etc.
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.