Glucose Dehydrogenase Activity Colorimetric Assay Kit

Glucose 1-dehydrogenase (NADᶧ) (EC 1.1.1.118 ) is an enzyme that catalyzes the chemical reaction: D-glucose + NADᶧ↔D-glucono-1,5-lactone + NADH + H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NADᶧ or NADPᶧ as acceptor. BioVision’s Glucose Dehydrogenase (GDH) Assay Kit provides a convenient tool for sensitive detection of the GDH in a variety of samples. The GDH present in sample will recognize D-glucose as a specific substrate leading to a proportional color development. The activity of GDH can be easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01 mU with our unit definition.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: This assay detects GDH activity as low as 0.01 mU with our unit definition.

• Simple procedure; takes ~ 30 minutes to 2 hours • Fast and convenient • Sensitive assays for measuring Glucose Dehydrogenase in various biological samples

Glucose Dehydrogenase

none

• GDH Assay Buffer • Glucose (2 M) • Developer (Lyophilized) • GDH Positive Control (Lyophilized) • NADH Standard (0.5 μmol, Lyophilized)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.