Alcohol Dehydrogenase Activity Colorimetric Assay Kit

Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NADᶧ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation. BioVision’s Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (λ = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: This assay detects ADH activity as low as 0.01 mU in samples.

• Simple procedure • Fast and convenient

Metabolism

none

• ADH Assay Buffer • Substrate • Developer (Lyophilized) • ADH Positive Control (Lyophilized) • NADH Standard (0.5 μmol, Lyophilized)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.