Glucose Oxidase Activity Colorimetric/Fluorometric Assay Kit

The glucose oxidase enzyme (GOx) (EC 1.1.3.4) is an oxido-reductase that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites. BioVision’s Glucose Oxidase Assay Kit provides a convenient tool for sensitive detection of the Glucose Oxidase in a variety of samples. The Glucose Oxidase in sample will recognize d-glucose as a specific substrate leading to proportional color development. The activity of GOx can be easily quantified colorimetrically (λ = 570 nm) or fluorometrically (Ex/Em = 535/585 nm). This assay detects GOx activity as low as 0.01mU with our unit definition.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em = 535/585 nm) • Species reactivity: Prokaryotes, Archaea etc. • GOx assay detects glucose oxidase activity as low as 0.01mU with our unit definition.

• Simple procedure • Fast and convenient • Sensitive assay for measuring glucose oxidase activity in various biological samples

Glucose Oxidase

none

• GOx Assay Buffer • OxiRed Probe (in DMSO) • GOx Substrate • GOx Developer (lyophilized) • GOx Positive Control (lyophilized) • H2O2 Standard (0.88 M)

-20°C

gel pack

12 months

see datasheet

Inquire

Cells and biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.