StayBrite™ Highly Stable Luciferase/Luciferin Reagent

Catalog number
K790-1000
Name
StayBrite™ Highly Stable Luciferase/Luciferin Reagent
Size
1000 assays
Price
834.00 EUR
Supplier

Details

Kit's description
BioVision’s Highly Stable Luciferase/Luciferin Reagent utilizes a genetically modified variant derived from the Luciferase of Diaphanes pectinealis (Chinese Firefly) endemic to Yunnan province, China. BioVision’s luciferase (rLucHS) has been modified to provide enhanced stability compared to the normal phenotype of Photinus pyralis, as well as a broader and more physiologically relevant effective pH range. At all pHs below ~8.2, rLucHS is significantly more active than commercial Photinus pyralis luciferase and is stable for weeks at room temperature and >60 minutes at 37°C. The specific activity of rLucHS is ~ 5x10ᶦᶦRLU/mg protein. This reagent has application to measure ATP levels in a wide variety of biological samples such as cells, tissues and fermentation broth, etc.
Highlights
• Detection method: Luminescence • Species reactivity: Mammalian • Kit size: Convenient sizes (100, 1000 and 10,000 assays) • Application: The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM).
Kit's benefits
• Simple procedure; takes ~ 20-30 minutes • Fast and convenient • Stable for >10 hours at room temperature and >60 minutes at 37°C.
Category
Luciferase
Kit's other name
none
Contents
• StayBrite™ Reconstitution Buffer • rLucHS Reagent Mix
Storage condition
-80°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture homogenates, fermentation broth