MMP-3 Inhibitor Screening Kit (Fluorometric)

The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development. In BioVision’s MMP-3 Inhibitor Screening Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Em/Ex=325/393 nm. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of MMP-3 inhibition. In addition, we also offer a human recombinant MMP-3 enzyme (Biovision #7783) and a MMP-3 Activity Assay Kit (Biovision #K783-100), separately.

• Detection method: Fluorescence (Ex/Em 325/393 nm) • Applications: The kit can be used to screen inhibitors of MMP:3.

• Simple procedure; takes ~ 35-45 minutes • Fast and convenient • The assay is simple, reliable and ensures accuracy and consistency when working with multiple samples.

MMP3

none

• MMP-3 Assay Buffer • MMP-3 Substrate • MMP-3 Enzyme (lyophilized) • Inhibitor Control (0.1 mM GM6001)

-20°C

gel pack

12 months

see datasheet

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Test inhibitors for MMP-3

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.