Renin Inhibitor Screening Kit (Fluorometric)

Catalog number
K799-100
Name
Renin Inhibitor Screening Kit (Fluorometric)
Size
100 assays
Price
611.00 EUR
Supplier

Details

Kit's description
Renin (EC 3.4.23.15), also known as an angiotensinogenase, is an enzyme that participates in the renin-angiotensin system (RAS) which mediates extracellular volume (i.e. blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. An over-active renin-angiotensin system leads to vasoconstriction and retention of sodium and water, causing hypertension. Renin inhibitors are widely used for the treatment of hypertension. BioVision’s Renin inhibitor screening Kit uses a synthetic peptide substrate with a fluorophore (EDANS) at one end and a quencher (DABCYL) at the other end. Renin catalyzes the cleavage of FRET substrate resulting in a product that is detected fluorometrically at Ex/Em = 328/552 nm. In the presence of a Renin inhibitor, the rate of hydrolysis of the substrate is decreased. The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Renin inhibitors also adaptable to a 384 well format.
Highlights
• Detection Method – Fluorescence (Ex/Em = 328/552 nm) • Application: : Screening potential inhibitors of renin : Characterize/study renin inhibitors
Kit's benefits
• Rapid & simple • Reliable test for screening potential inhibitors of renin • Includes Inhibitor Control [Aliskiren]
Category
Renin
Kit's other name
Angiotensinogenase Inhibitor Screening Kit (Fluorometric)
Contents
• Renin Assay Buffer • Renin Substrate • Active Human Renin (lyophilized) (15 µg) • Inhibitor Control [Aliskiren] (200 µM)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.