Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit

Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. AHCY regulates the intracellular SAH concentration which in turn regulates S-adenosyl methionine (SAM)-dependent methyltransferases. Down-regulation of AHCY has been associated with certain forms of cancer and Huntington’s disease, while in Wilson’s disease; the enzyme is inhibited by the accumulated copper. Mutations in the AHCY gene cause SAHH deficiency disease. BioVision’s AHCY Activity Assay kit can kinetically measure AHCY activity by detecting adenosine generation resulting from the hydrolysis of SAH. Adenosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the OxiRed™ Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification (L.O.Q) is 1 µU recombinant human AHCY.

• Detection Method – Fluorescence (Ex/Em = 535/587 nm) • Application: Detection of AHCY activity

• Rapid, simple & convenient • The assay can detect AHCY activity as low as 1 µU recombinant human AHCY.

Adenosylhomocysteinase, AHCY, S-adenosylhomocysteine hydrolase, SAHH

S-adenosylhomocysteine hydrolase (SAHH) Activity Fluorometric Assay Kit, S-adenosylhomocysteine synthase Activity Fluorometric Assay Kit, Adenosylhomocysteine hydrolase Activity Fluorometric Assay Kit

• AHCY Assay Buffer • Homogenization Buffer • OxiRed™ Probe • SAH Substrate • Enzyme Mix I • Enzyme Mix II • Adenosine Standard (10 mM) • AHCY Enzyme (Positive Control)

-20°C

gel pack

12 months

see datasheet

Detection of AHCY activity

• Purified recombinant protein • Cell and tissue lysate

Biovision supplies other types of Assays as 1.