BCA Protein Assay Kit II

Catalog number
K813-2500
Name
BCA Protein Assay Kit II
Size
2500 assays
Price
176.00 EUR
Supplier

Details

Kit's description
Biovision’s BCA Protein Assay kit is useful for colorimetric detection and quantification of total protein content even in the presence of detergents. The Kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in an alkaline medium. The amount of purple-colored product formed during the reaction can be correlated to the amount of protein in the assay. The Cu+1-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a wide range of protein concentrations between 25-2000 μg/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard. This BCA Protein Assay Kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of the samples.
Highlights
• Detection method: Absorbance (562 nm) • Detergent compatible
Kit's benefits
• Simple & convenient • High linearity: linear over a wide range of protein concentrations between 25-2000 μg/ml.
Category
Protein Quantitation
Kit's other name
none
Contents
• BCA Reagent A • BCA Reagent B • BSA Standard (2 mg/ml)
Storage condition
RT
Shipping condition
RT
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
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Biovision supplies other types of Assays as 1.