BCA Protein Assay Kit (Test Tube) - Reducing Agent Compatible

Catalog number
K819-250
Name
BCA Protein Assay Kit (Test Tube) - Reducing Agent Compatible
Size
250 assays
Price
328.00 EUR
Supplier

Details

Kit's description
BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is the only protein estimation kit on the market that is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM & β-mercaptoethanol up to 35 mM. This kit is adapted from BioVision’s BCA Assay Kit II (cat. # K813) and is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. Reducing agents in samples interfere with BCA assay due to their ability to reduce Cu+2. BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is useful for determining protein concentrations of samples containing DTT, TCEP & β-mercaptoethanol. The assay is linear over the widest range of protein concentration between 25-2000 μg/ml. In general, protein concentrations are calculated with reference to a commonly used protein standard. The kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.
Highlights
• Detection method: Absorbance (562 nm) • Reducing agent compatible
Kit's benefits
• Simple & convenient. • Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & β-mercaptoethanol (35 mM). • The assay is linear over a wide range of protein concentration (25-2000 μg/ml).
Category
Protein Quantitation
Kit's other name
none
Contents
• BCA Reagent A • BCA Reagent B • BSA Standard (2 mg/ml) • Blocking Reagent (20 mg/tube) • Blocking Reagent Buffer
Storage condition
RT
Shipping condition
RT
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Measuring total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Samples tested
pure proteins, cell/tissue extracts or lysates
Test
Biovision supplies other types of Assays as 1.