N′-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit

N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy. Biovision’s NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe generating enhanced fluorescence signal that can be measured at Ex/Em = 392/482 nm. In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.

• Detection method: Fluorescence (Ex/Em = 392/482 nm) • Species reactivity: Mammalian • Application : Screening/studying/characterizing potential inhibitors of NNMT

• Rapid assay and simple protocol • Reliable test for screening potential inhibitors of NNMT • Includes Inhibitor Control, 1-Methylnicotinamide (MNA)

NNMT, Nicotinamide N-Methyltransferase

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• NNMT Assay Buffer • NNMT Enzyme • S-Adenosylmethionine (SAM) • Nicotinamide • Enzyme-I • Enzyme-II • 1-Methylnicotinamide (MNA) (150 mM) • Thiol Detecting Probe (DMSO) • SAM Reconstitution Buffer

-80°C

dry ice

12 months

Mammalian

Screening/studying/characterizing potential inhibitors of NNMT

Biovision supplies other types of Assays as 1.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.