SuperBrite™ ECL Western Blot Substrate/Detection Kit

BioVision’s SuperBrite™ ECL kit includes second-generation ECL substrates. They are highly sensitive, non-radioactive, and enhanced luminol-based chemiluminescent substrates for easy detection of horseradish peroxidase (HRP) on immunoblots. Blots can be repeatedly exposed to X-ray film or a CCD camera to obtain optimal results or stripped off the immune-detection reagents and re-probed.

The most sensitive ECL Western blotting substrate kit on the market. Application: Western Blotting, Dot Blotting.

50 ml SuperBrite™ ECL Western Blotting solutions, sufficient for 30 min-gel blots or 2500 cm2 of membrane III. Advantages: • Minimal volumes required: Can be used for 30 mini-gel blots or 2500 cm2 of membrane. • Exceptional Sensitivity: Detects targets in the low picogram levels. • Multiple imaging options: Compatible with X-Ray, CCD and laser based imaging. • Ready to Use: No additional reconstitutions required. • Stable signal: Signals developed are stable. • Economical: Available at the most competitive rates.

Western Blotting Substrate

Western blot ECL substrate kit

Reagent A Reagent B

+4°C

gel pack

12 months

The most sensitive ECL Western blotting substrate kit on the market. Application: Western Blotting, Dot Blotting.

Western blot. Dot blot

pure proteins, cell/tissue extracts or lysates

Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.

The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.