HIV-1 Protease Activity Assay Kit (Fluorometric)

Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of the virus as it cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Without effective HIV1 protease, HIV virions remain non-infectious. BioVision’s HIV-1 Activity assay kit utilizes the ability of active HIV-1 protease to cleave a synthetic peptide substrate to release the free fluorophore which can be easily quantified (Ex/Em = 330/450 nm) using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect HIV-1 protease activity in samples and of purified enzyme.

Sensitive fluorometric assay to detect HIV:1 Protease activity.

Simple, sensitive assay. Fluorometric detection

Protease activity

HIV-1 Retropepsin activity assay kit, cd05482 activity assay kit

HIV-1 Protease Assay Buffer HIV-1 Protease Dilution Buffer HIV-1 Protease Substrate HIV-1 Protease (Positive Control) Fl. Standard (10 mM in DMSO)

-80°C

dry ice

12 months

Sensitive fluorometric assay to detect HIV:1 Protease activity.

• Detect activity of recombinant HIV-1 protease • Determine activity of purified HIV-1 protease

Proteins/lysates

hiv

Biovision supplies other types of Assays as 1.

The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome(AIDS). AIDS is a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, pre-ejaculate, or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. recombinant HIV 1 and 2 gag gene proteins p24, p17, p55 immunodominant epitopes and envelope glycoproteins, gp120 are used for production of diagnostic detection antibodies.