HIV-1 Protease Inhibitor Screening Kit (Fluorometric)

Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of the virus as it cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Without effective HIV-1 protease, HIV virions remain non-infectious. BioVision’s HIV-1 Protease Inhibitor Screening kit utilizes the ability of active HIV-1 protease to cleave a synthetic peptide substrate resulting in the release of free fluorophore, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a HIV-1 Protease inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HIV-1 Protease.

The simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HIV:1 Protease with a fluorometer or fluorescence microplate reader.

Simple and High throughput adaptable Detect using a fluorometer or fluorescence microplate reader.

Protease activity

HIV-1 Retropepsin inhibitor screening kit, cd05482 inhibitor screening kit

HIV-1 Protease Assay Buffer HIV-1 Protease Dilution Buffer HIV-1 Protease Substrate HIV-1 Protease HIV-1 Protease Inhibitor (1 mM Pepstatin A in DMSO)

-80°C

dry ice

12 months

The simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HIV:1 Protease with a fluorometer or fluorescence microplate reader.

• Screening/studying/characterizing inhibitors of HIV-1 Protease

Variable: Inhibitors, small molecules, proteins, etc.

hiv

The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome(AIDS). AIDS is a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, pre-ejaculate, or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. recombinant HIV 1 and 2 gag gene proteins p24, p17, p55 immunodominant epitopes and envelope glycoproteins, gp120 are used for production of diagnostic detection antibodies.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.