Beta Galactosidase (β-Gal) Inhibitor Screening Kit (Fluorometric)

Beta Galactosidase (β-Gal, EC: 3.2.1.23) is an enzyme which hydrolyzes the β-galactosides into monosacchanides. β-Gal is widely used as a reporter gene in the field of molecular biology. Senescence Associated β-Gal (SA-β-Gal) is an isoform of β-Gal which has the optimal activity at pH 6.0, and is mostly used as a biomarker for senescent cells (K802). β-Gal is an essential enzyme in humans and its deficiency results in Morquio’s Syndrome, a severe birth defect. β-Gal can also be used as a tool to study protein-protein interaction. In BioVision’s Beta-Galactocidase Inhibitor Screening Kit, β-Gal converts β-Gal substrate to give an intensely fluorescent product (Ex/Em = 480/520 nm). In the presence of a β-Gal inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of fluorescence. This assay kit can be used to screen/study/characterize the potential inhibitors of Beta Galactosidase. The assay is simple, high-throughput adaptable and can be performed within 30 min.

A simple and high:throughput adaptable assay kit used to fluorometrically (Ex/Em = 480/520 nm) screen/study/characterize potential inhibitors of beta galactosidase within 30 min.

Simple and High throughput adaptable 30 minutes short protocol Detect using a fluorometer or fluorescence microplate reader.

Beta Galactosidase

Elastin receptor 1 assay kit

β-Gal Assay Buffer β-Gal Substrate (in DMSO) β-Galactosidase β-Gal Inhibitor Control

-20°C

gel pack

12 months

A simple and high:throughput adaptable assay kit used to fluorometrically (Ex/Em = 480/520 nm) screen/study/characterize potential inhibitors of beta galactosidase within 30 min.

• Screening/characterizing/studying potential inhibitors of Beta-Galactosidase

Variable: Inhibitors, small molecules, proteins, etc.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.