Hexokinase (HK) Inhibitor Screening Kit (Colorimetric)

Hexokinases (HK) play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km. Hexokinase deficiency leads to severe human diseases such as X-linked muscular dystrophy and a rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various tumors and is associated with metastasis. In BioVision’s Hexokinase Inhibitor Screening kit, glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. In the presence of a Hexokinase inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of absorbance. This assay kit can be used to screen/study/characterize the potential inhibitors of Hexokinase. The assay is simple, high-throughput adaptable and can be performed within 30 min.

A simple and high:throughput adaptable assay kit used to colorimetrically (450 nm) screen/study/characterize potential inhibitors of Hexokinase within 30 min.

Simple and High throughput adaptable Colorimetric Detection Screen within 30 min

Hexokinase

none

HK Assay Buffer HK Substrate HK Coenzyme HK Converter HK Developer Hexokinase HK Inhibitor Control

-20°C

gel pack

12 months

A simple and high:throughput adaptable assay kit used to colorimetrically (450 nm) screen/study/characterize potential inhibitors of Hexokinase within 30 min.

• Screening/characterizing/studying potential inhibitors of Hexokinase

Variable: Inhibitors, small molecules, proteins, etc.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.