EZLabel™ Antibody FITC Labeling Kit

BioVision’s EZLabel Antibody-FITC Labeling Kit provides an easy way to label antibodies with Fluorescein Isothiocyanate (FITC) in a user-friendly spin column format. FITC is an ideal dye for fluorescent labeling of antibodies among the most commonly used fluorescent dyes for labeling antibodies. Each spin column provided in the kit can be used to label up to 1 mg of the target antibody. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of antibody per reaction. FITC-labeled antibody has an excitation and emission wavelengths at 494 nm and 520 nm respectively, and can be directly used for downstream applications including ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis, etc.

Easy to use Kit to label upto 5 mg of Antibody with FITC for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis

All necessary reagents included. Fast and efficient labeling of antibodies with FITC

Antibody labeling

none

EZLabel FITC EZLabel Spin Column EZLabel Quenching Buffer EZLabel Elution Buffer

+4°C

gel pack

12 months

Easy to use Kit to label upto 5 mg of Antibody with FITC for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis

Labeled antibodies can be used for ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis

Antibody

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.

Anti-FITC Antibody

anticorps