EZLabel™ Protein FITC Labeling Kit

BioVision’s EZLabel™ Protein-FITC Labeling Kit provides an easy way to label proteins with Fluorescein Isothiocyanate (FITC) in a user-friendly spin column format. FITC is an ideal dye for fluorescent labeling of proteins among the most commonly used fluorescent dyes for labeling proteins. Each spin column provided in the kit can be used to label up to 1 mg of the target protein. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of protein per reaction. FITC-labeled protein has an excitation and emission wavelengths at 494 nm and 520 nm respectively, and can be directly used for downstream applications including ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis, etc.

Easy to use Kit to label upto 5 mg of Protein with FITC for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis

All necessary reagents included. Fast and efficient labeling of proteins with FITC

Protein labeling

none

EZLabel FITC EZLabel Spin Column EZLabel Quenching Buffer EZLabel Elution Buffer

+4°C

gel pack

12 months

Easy to use Kit to label upto 5 mg of Protein with FITC for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis

Labeled proteins can be used for ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis

Proteins

This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.

Anti-FITC Antibody