EZLabel™ Antibody Biotin Labeling Kit

BioVision’s EZLabel™ Antibody-Biotin Labeling Kit provides an easy way to label antibodies with Biotin in a user-friendly spin column format. Due to its high affinity for tetrameric avidin proteins, biotin is ideal for labeling antibodies. Each pair of spin columns provided in the kit can be used to purify up to 1 mg of the labeled target antibody. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of antibody per reaction. The biotin-labeled antibody can be directly used for multiple downstream applications including ELISA, western blot, Immunohistochemistry, Immunoassays, etc.

Easy to use Kit to label upto 5 mg of Antibody with Biotin for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis

All necessary reagents included. Fast and efficient labeling of antibodies with Biotin

Antibody labeling

none

EZLabel™ Biotin EZLabel™ Spin Column EZLabel™ Elution Buffer

+4°C

gel pack

12 months

broad

Labeled antibodies can be used for ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis

Antibody

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Biotinylated

anticorps