TEV Protease Inhibitor Screening Kit (Fluorometric)

TEV Protease (EC: 3.4.22.44) is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). TEV Protease has high specificity and great stability and is active over a wide range of temperatures (4-37°C) with an optimal activity at 34°C. BioVision’s Inhibitor Screening Kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). In the presence of a TEV Protease-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of TEV Protease.

Markets's first and only assay kit to fluorometrically (Ex/Em = 490/560 nm) screen/study/characterize potential inhibitors of TEV Protease. HTP adaptable.

Simple, rapid assay HTP Adaptable Fluorometric Detection

Proteases

none

TEV Protease Assay Buffer TEV Protease TEV Protease Substrate TEV Protease Inhibitor (2 mM)

-20°C

gel pack

12 months

Markets's first and only assay kit to fluorometrically (Ex/Em = 490/560 nm) screen/study/characterize potential inhibitors of TEV Protease. HTP adaptable.

• Screening/studying/characterizing inhibitors of TEV Protease.

Chemical libraries, small molecules

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.