MMP-9 Inhibitor Screening Kit (Fluorometric)

Matrix metalloproteinase (MMP) family proteins are involved in the breakdown of extracellular matrix, and have been recognized as potential targets for a variety of pathologies. Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B, is the mostly studied MMP, due to its fundamental roles in cancer biology, autoimmune disease, and other conditions. MMP-9 is a metalloenzyme, composed of a pro-domain, a gelatin binding domain, a catalytic domain, a proline rich linker region, and a carboxyl terminal hemopexin like domain. This enzyme degrades various substrates including gelatin, collagen types IV / V, and elastin. BioVision’s MMP-9 Inhibitor Screening Kit provides a quick and sensitive way for screening, studying and characterizing potential inhibitors of MMP-9. In this assay, MMP-9 hydrolyzes a FRET-based MMP-9 substrate and releases the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm.

A Fluorometric Assay Kit (Ex/Em=325/393 nm) to screen/study/charecterize MMP:9 inhibitors easily. 100 assays

Simple, fast assay. Fluorometric detection.

MMP

Gelatinase B Inhibitor Screening Kit, Type IV Collagenase Inhibitor screening kit

MMP-9 Assay Buffer MMP-9 Substrate (2 mM) MMP-9 (lyophilized) Inhibitor (NNGH 1 mM)

-20°C

gel pack

12 months

A Fluorometric Assay Kit (Ex/Em=325/393 nm) to screen/study/charecterize MMP:9 inhibitors easily. 100 assays

• Screening/studying/characterizing potential MMP-9 inhibitors

Chemical libraries, small molecules

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.