HIV-2 Protease Activity Assay Kit (Fluorometric)

Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). Infection with HIV-2 virus causes immunosuppression, as well as AIDS, characterized by the same signs, symptoms and opportunistic infections as HIV-1. However, HIV-2-associated AIDS may often be associated with lower viral load levels than HIV-1. Aspartyl HIV-2 Protease (retropepsin) is an essential enzyme for the life-cycle of HIV virus. Expressed as a part of the Gag-Pol polyprotein complex in the infected cells, it is auto-catalytically released upon formation of an immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins resulting in the maturation of the virus. In HIV-infected patients the enzyme is subjected to intensive mutagenesis and mutants resistant to applied medicines are produced as a result of the selection pressure. BioVision’s HIV-2 Protease Activity assay kit utilizes the ability of active HIV-2 Protease to cleave a synthetic peptide substrate to release the free fluorophore which can be easily quantified (Ex/Em = 330/450 nm) using a fluorometer or fluorescence microplate reader. This assay is simple, rapid and can detect HIV-2 protease activity in samples and of purified enzyme.

Sensitive fluorometric assay to detect HIV:2 Protease activity.

Simple, sensitive assay. Fluorometric detection

Protease activity

HIV-2 retropepsin activity assay kit, cd05482 activity assay kit

HIV-2 Protease Assay Buffer HIV-2 Protease Dilution Buffer HIV-2 Protease Substrate HIV-2 Protease (Positive Control) Fl. Standard (10 mM in DMSO)

-80°C

dry ice

12 months

Sensitive fluorometric assay to detect HIV:2 Protease activity.

• Detect activity of recombinant HIV-2 protease • Determine activity of purified HIV-2 protease

Proteins/lysates

hiv

Biovision supplies other types of Assays as 1.

The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome(AIDS). AIDS is a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, pre-ejaculate, or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. recombinant HIV 1 and 2 gag gene proteins p24, p17, p55 immunodominant epitopes and envelope glycoproteins, gp120 are used for production of diagnostic detection antibodies.