MMP-14 Inhibitor Screening Kit (Fluorometric)

The Matrix metalloproteinase-14 (MMP-14) (MT1-MMP) belongs to a family of zinc-dependent endopeptidases that are responsible for the degradation of the extracellular matrix (ECM) proteins including collagens, elastins, gelatin, matrix glycoproteins and proteoglycans, during normal development and disease processes. MMPs can be classified into at least four different families of closely related members - collagenases, gelatinases, stromelysins, and membrane-type MMPs (MT-MMPs). MMP-14 belongs to the family of MT-MMPs and is localized on cell surfaces via its transmembrane domain. MMP-14 is expressed in adult lung, placenta, kidney, ovaries, intestine, prostate and spleen along with certain carcinomas. The ability of MMP-14 to degrade type I collagen, and activate pro-MMP-2 and pro-MMP-9 makes it a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. BioVision’s MMP-14 Inhibitor Screening Kit provides a simple, fast and high-throughput adaptable method to screen/study/characterize potential MMP-14 inhibitors. The assay utilizes the ability of MMP-14 to cleave a synthetic MCA-based peptide substrate to release free MCA (7-Methoxycoumarin-4-acetic acid), which can be easily quantified using a fluorometer or fluorescence microplate reader at Ex/Em = 325/420 nm. In the presence of a MMP-14 specific inhibitor, the cleavage of the substrate is reduced/abolished, resulting in decrease or total loss of the MCA fluorescence.

A Fluorometric Assay Kit (Ex/Em=325/420 nm) to screen/study/charecterize MMP:14 inhibitors easily. 100 assays

Simple, fast assay. Fluorometric detection.

MMP

Matrix metalloproteinase-14 Inhibitor Screening Kit, MT1-MMP Inhibitor Screening Kit

MMP-14 Assay Buffer MMP-14 Substrate (4 mM) MMP-14 Enzyme MMP-14 Inhibitor (NNGH, 2 mM)

-20°C

gel pack

12 months

A Fluorometric Assay Kit (Ex/Em=325/420 nm) to screen/study/charecterize MMP:14 inhibitors easily. 100 assays

• Screening/studying/characterizing potential MMP-14 inhibitors

Chemical libraries, small molecules

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.