EZQuant™ dsDNA Quantitation Kit (Fluorometric)

Accurate and sensitive quantification of double stranded DNA (dsDNA) has wide applications. The major disadvantages of traditional absorbance based method for DNA quantitation include (1) lower sensitivity, (2) inability to distinguish between DNA and RNA, (3) interference due to contaminants commonly found in nucleic acid preparations and (4) relatively large contribution of nucleotides and single-stranded nucleic acid to the signal. BioVision’s dsDNA Quantitation Kit is sensitive, non-radioactive and can specifically detect dsDNA in the presence of ssDNA, RNA, and free nucleotides. The assay is based on the fluorescent dsDNA-binding dye that specifically binds to dsDNA to generate fluorescence (Ex/Em = 480/530 nm), which enables the sensitive quantitation of small amounts of dsDNA (as low as 40 pg). This highly sensitive assay is ready as supplied for 96-well plate format and can be adapted to 384-well plate.

• Detection method: Fluorescence (Ex/Em = 480/530 nm) • Application: Quantitation of dsDNA from viral DNA, miniprep DNA, genomic DNA, PCR amplification products etc.

• Non-radioactive • Highly sensitive • Specifically detects dsDNA in the presence of ssDNA, RNA, and free nucleotides

dsDNA Quantitation, DNA Quantitation

none

• TE Buffer (20X) • dsDNA Dye (200X) • dsDNA Standard (100 µg/ml)

-20°C

gel pack

12 months

see datasheet

Quantitation of dsDNA from viral DNA, miniprep DNA, genomic DNA, PCR amplification products etc.

dsDNA sample in solution