PCR Quick Screening Kit, InsertFinder™

Identification of clones that contain the desired DNA insert is an essential step in molecular cloning procedure. Traditional methods for identifying the correct DNA inserts require growing up individual colonies, isolating plasmid DNAs, and then digesting with restriction enzymes to analyze each individual clone. Such traditional procedures are very time consuming, laborious, and expensive. The new InsertFinderTM PCR Quick Screening Kit is designed to quickly screen clone candidates using PCR technology. The procedure allows screen of colonies directly from plate, without growing up the cultures. The proprietary Cell Lysis Buffer provided with the kit can efficiently lyse cells (bacteria or yeast) without interfering with PCR reactions. The PCR Enhancer included in the kit allows amplification of hairpin structure or GC-rich region efficiently. PCR products can directly be analyzed on an agarose gel without enzyme digestions. The kit provides a simple, convenient, and economical way to identify correct inserts. It is also suitable for screening large numbers of colonies.

• Application: Kit is designed to quickly screen large number of cloning inserts

• Procedure is quick and simple • Clones can be analyzed directly on an agarose gel

Clone Screening

none

• InsertFinder Lysis Buffer • 10X PCR Buffer • dNTP • Taq DNA Polymerase • PCR Enhancer

-20°C

gel pack

12 months

see datasheet

Inquire

Bacteria and yeast

PCR, polymerase chain reaction

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.