EZCellTM Cell Invasion Assay (Basement Membrane), 96-well, 8 µm

Cell invasion is the ability of cells to migrate from one area to another through an extracellular matrix. Cell invasion is exhibited by both normal cells as well as cancerous cells in response to specific external signals, including chemical & mechanical stimuli. During invasion, extracellular matrix is enzymatically degraded by cellular proteases before cells migrate to the new location. Cell invasion is required for normal processes such as wound repair, vasculature formation and the inflammatory response as well as the abnormal invasion of tissues by tumor cells during metastasis. BioVision’s Cell Invasion Assay Kit utilizes a Boyden chamber coated with Basement Membrane Extract (BME), where the cells invade the matrix and then migrate through a semipermeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds. The percent cell invasion can be analyzed directly in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.

• Detection method : Fluorescence (Ex/Em = 530/590 nm) • Applications: Measure cell invasion in response to stimuli. : Screen, study, or characterize compounds that influence cell invasion.

• Highly sensitive fluorometric method to measure cell invasion in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell invasion

Cell Invasion

none

• Cell Invasion Chamber • Basement Membrane Solution • Control Invasion Inducer • Cell Dissociation Solution • Wash Buffer • Cell Invasion Dye

-20°C

gel pack

12 months

see datasheet

• Measure cell invasion in response to stimuli. • Screen, study, or characterize compounds that influence cell invasion.

Invasive cell lines

cell

Biovision supplies other types of Assays as 1.

For cells, cell lines and tissues in culture till half confluency.Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.