Cell Transformation Assay Kit (Colorimetric)

The transformed cells can proliferate without attaching to surface. Anchorage independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). BioVision’s Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit is based on the conversion of the tetrazolium salt (WST-8) to formazan by cellular mitochondrial dehydrogenases. The generated signal is directly proportional to the number of living cells. This one-step method is non-radioactive and simple (just add-and-read, does not require harvesting cells, and solubilization steps). The assay is high-throughput adaptable and has wide linear range from 10000-400000 cells. The entire Cell Transformation Assay can be finished within 7-8 days.

• Detection method : Colorimetric (OD 450 nm) • Applications: Measure cell transformation in response to stimuli. : Screen, study, or characterize compounds that influence cell transformation. : HTP adaptable

• Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation

Cell-based Assays

none

Agarose Powder DMEM Solution (10X) Staining Solution WST Reagent Electro Coupling Solution (ECS)

-20°C

gel pack

12 months

Mammalian cells

• Measure cell transformation in response to stimuli • Screen and characterize compounds that influence cell transformation

Adherent or Suspension Cells

cell

Biovision supplies other types of Assays as 1.

For cells, cell lines and tissues in culture till half confluency.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.