Cell Transformation Assay Kit (Fluorometric)

Transformed cells can proliferate without attaching to surface Anchorage-independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). BioVision’s Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit uses a quantitative dye that binds to nucleic acid and generates green fluorescence. This one-step method is non-radioactive and simple (just add-and-read, and does not require tedious labor such as counting colonies). The assay is high-throughput adaptable and has wide linear range from 50-60,000 cells. The entire assay can be finished within 7-8 days.

• Detection method : Fluorescence (Ex/Em = 480/530 nm) • Applications: Measure cell transformation in response to stimuli. : Screen, study, or characterize compounds that influence cell transformation. : HTP adaptable

• Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation

Cell-based Assays

none

Agarose Powder DMEM Solution (10X) Staining Solution Agarose Solubilization Solution Quantitative Dye (200X)

-20°C

gel pack

12 months

Mammalian cells

• Measure cell transformation in response to stimuli • Screen and characterize compounds that influence cell transformation

Adherent or Suspension Cells

cell

Biovision supplies other types of Assays as 1.

For cells, cell lines and tissues in culture till half confluency.