Human βIG-H3 ELISA Kit

BioVision’s Human βIG-H3 (also known as Kerato-epithelin, RGDCAP) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human βIG-H3 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human βIG-H3 coated on a 96-well plate. Standards and samples are pipetted into the wells and βIG-H3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human βIG-H3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of βIG-H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

• Detection method: Absorbance (550 nm) • Species reactivity: Human

• Easy, convenient and time-saving method to assay for human βIG-H3 • The minimum detectable dose of βIG-H3 is typically less than 25 pg/ml. • This ELISA kit shows no cross-reactivity with the rmβIGH3. The inter assay reproducibility CV is

Protein Quantitation

none

• βIG-H3 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human βIG-H3. • Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. • Standards (Item C): 2 vials of recombinant human βIG-H3. • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. • Detection Antibody βIG-H3 (Item F): 2 vial of biotinylated anti-human βIG-H3 (each vial is enough to assay half microplate). • HRP-Streptavidin Concentrate (Item G): 200 µl 300x concentrated HRP-conjugated streptavidin. • TMB One-Step Substrate Reagent (Item H): 12 ml of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffer solution. • Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid.

-20°C

gel pack

12 months

see datasheet

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Serum, Plasma, Cell culture supernatants and urine

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED