Protein Disulfide Isomerase (PDI) Activity Assay (Fluorometric)

Protein Disulfide Isomerases (PDI, EC: 5.3.4.1) are multifunctional proteins that constitute a thio-disulfide oxidoreductase family. PDI is abundant in the lumen of endoplasmic reticulum (ER). PDI plays an essential role in catalyzing the rearrangement of disulfide (S-S) bonds in proteins and functions as a chaperone by inhibiting protein aggregation. Recent studies show that PDI activity is essential for cancer cell survival and proliferation, and targeting PDI activity with specific inhibitors abrogates survival responses leading to ER stress in cancer cells. Therefore, the detection of PDI activity is critical for mechanistic study, diagnosis, prevention, and therapeutic purposes. BioVision’s PDI Activity Assay kit utilizes the reductase activity PDI for determining its activity in a variety of samples. The Kit employs a synthetic quenched fluorescent substrate which gets cleaved in the presence of PDI producing an enhanced fluorometric signal (Ex/Em = 490/580 nm). In the presence of BioVision’s PDI specific inhibitor, the quenched fluorescent substrate cannot be cleaved by PDI. BioVision’s PDI Activity Assay Kit is simple, sensitive, and high-throughput adaptable with the detection limit as low as 0.1 mU.

• Detection method: Fluorescence (Ex/Em= 490/480 nm) • Species reactivity: Mammalian • Application: This assay has a detection sensitivity as low as 0.1 mU.

• Simple procedure • Fast and convenient

Metabolism

none

• PDI Assay Buffer • PDI Substrate • PDI Activator (100 mM) • PDI Inhibitor (50X) • PDI Positive Control • Fluorescence Standard (25 µM)

-20°C

gel pack

12 months

Mammalian

This assay has a detection sensitivity as low as 0.1 mU.

Cell and tissue culture lysates and purified enzyme preparations.

Biovision supplies other types of Assays as 1.