EZScreen™ Glucose Colorimetric Assay Kit (384 Well)

Glucose (C6H12O6, FW: 180.16) is delivered to the cells from the blood stream as the primary biological fuel to generate the universal energy molecule, ATP. Even though human body has evolved an advanced metabolic pathway to keep the blood glucose level in normal range, failure happens and that leads to develop conditions of persistent high or low blood sugar. Diabetes mellitus is one such most important metabolic disorder where an increased blood-sugar level develops compare to normal blood sugar level. Glucose level in blood is also a key diagnostic parameter for many metabolic disorders and its measurement is very important in both research and drug discovery. BioVision’s EZScreen™ Glucose Colorimetric Assay Kit, uses a glucose enzyme mix which oxidizes glucose specifically and produces a product which reacts with a chromophore generating a stable signal (OD: 590 nm). This color is directly proportional to the amount of glucose present in the sample. The method is quantitative, rapid, simple, sensitive, and designed to be used in high throughput settings. The kit can detect 0.5 to 5 mM of glucose in various biological samples

• Detection method: Absorbance (590 nm) • Species reactivity: Mammalian • Applications: The kit detects 0.5:500 µM glucose samples

• Simple procedure; takes ~40 minutes • Fast and convenient • Kit provides all necessary buffers and reagents for a accurate assay of glucose in various mammalian samples.

Glucose Assay

none

• EZScreen™ Glucose Assay Buffer • EZScreen™ Glucose Probe • EZScreen™ Glucose Enzyme Mix (lyophilized) • EZScreen™ Glucose Standard (100 mM)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids and growth medium

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.