EZScreen™ Lactate Dehydrogenase Activity Colorimetric Assay Kit (384-well)

Lactate dehydrogenase (LDH) is an oxidoreductase (EC 1.1.1.27) present in a wide variety of organisms. LDH catalyzes the interchange of lactate to pyruvate, with the concomitant interconversion of NAD+ to NADH. LDH is present in many organs and tissues including liver, heart, pancreas, kidneys, skeletal muscles, brain, and blood cells. When illness or injury damages cells, LDH may be released into the bloodstream, causing a rise of LDH level in blood. Elevated LDH in serum can indicate a number of unfavorable medical conditions like cerebral attack, heart attack, hepatitis, muscular dystrophy, hemolytic anemia and even certain cancers. Therefore, quantitation of LDH activity has significant clinical importance. BioVision’s EZScreenTM Lactate Dehydrogenase Colorimetric Assay kit, offers a simple and sensitive assay that can detect and quantify the enzymatic activity of LDH. In this assay, LDH oxidizes a substrate utilizing NAD as cofactor. The reduced cofactor (NADH) reacts with a chromophore producing a stable color (OD: 500 nm) that is directly proportional to LDH activity. The assay is suitable to detect the enzymatic activity as low as 0.08 mU in various biological samples

• Detection method: Absorbance (500 nm) • Species reactivity: Mammalian • Application: The kit can as low as 0.08 mU of LDH directly in samples.

• Simple procedure; takes ~ 30 minutes • Fast and convenient • Sensitive assays for measuring LDH activity in various biological samples. The kit has been optimized for high throughput analyses

LDH

none

• LDH Assay Buffer • LDH Substrate Mix (lyophilized) • NADH Standard (lyophilized) • LDH Positive Control (lyophilized)

-20°C

gel pack

12 months

Mammalians

• Measure triglycerides in biological samples • Screen and characterize compounds that influence triglyceride metabolism

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.