EZScreen™ Free Fatty Acid Colorimetric Assay Kit (384-well)

Fatty Acids play important roles in normal metabolism and in many disease states. They are precursors to a number of bioactive classes of compounds including prostaglandins and leukotrienes, and have been implicated to play roles in such diverse functions as the immune system, autism, and the inflammatory response. Fatty acid levels are an important measure of the physiologic state. High levels are associated with diabetes mellitus, obesity, endocrine dysfunctions, and metabolic syndrome. BioVision’s EZScreenTM Free Fatty Acid Colorimetric assay kit provides a simple method for the quantification free fatty acids. The kit offers a convenient, sensitive, enzyme-based method for the detection of long-chain (C-8 (octanoate) and longer) fatty acids in various biological samples. In the EZScreenTM assay, fatty acids are converted to their CoA derivatives, which are subsequently oxidized by the enzyme mix to yield an intermediate. Formed intermediate then reacts with the Free Fatty acid probe to generate color which can be read by a spectrophotometer at OD 570 nm. Our 384-well format enables analyses of large number of samples on a single plate in a high throughput mode. Sample volumes between 1 and 10 μl can be used with this kit

• Detection method: Absorbance (570 nm) • Species reactivity: Mammalian • Application: The kit has can detect physiological concentration of Free Fatty Acids in samples

• Simple procedure; takes ~40 minutes • Fast and convenient • Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples

Fatty Acid Assay

none

• Fatty Acid Assay Buffer • Fatty Acid Probe (in DMSO, anhydrous) • ACS Reagent (lyophilized) • Enzyme Mix (lyophilized) • Enhancer • Palmitic Acid Standard (1nmol/µl)

-20°C

gel pack

12 months

Species reactivity- Mammalian

The kit has can detect physiological concentration of Free Fatty Acids in samples

Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.