EZScreen™ NAD+/NADH Colorimetric Assay Kit (384-well)

Nicotinamide Adenine Dinucleotide (NAD) is a co-enzyme indispensible for the activity of multiple catalytic proteins. NAD+ and NADH, its reduced form, participate in many biological redox reactions. NAD+ is mostly associated with redox enzymes required for catabolic reaction and energy production. BioVision’s NADH/NAD Quantification Kit provides a convenient tool for a sensitive detection of NADH, NAD and their ratio. There is no requirement to purify NADH or NAD from samples. The NADH selective developer develops color while reacting with NADH and can be detected at OD 450 nm. The total NAD can be easily quantified by comparing with the provided NADH standard. The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The kit can detect as low as 0.04 pmol of NADH in a 384 well assay plate. The reaction specifically detects NADH and NAD, but not NADP nor NADPH.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio.

• Simple procedure; takes ~2 hours • Fast and convenient • Kit contains the necessary reagents for accurate measurement of NAD and NADH and their ratio.

NAD/NADH

none

• NADH/NAD Extraction Buffer • NAD Cycling Buffer • NAD Cycling Enzyme Mix • NADH Developer • Stop Solution • NADH Standard

-20°C

gel pack

12 months

Species reactivity- Mammalian

BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio

Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.