EZScreen™ Glycogen Colorimetric Assay Kit (384-Well)

Glycogen is a branched polysaccharide, made of glucose units that serves as the primary source of energy storage in animals. Most of the glucose residues in glycogen are linked by α-1,4-glycosidic bonds with branching via α-1,6 linkage. In mammals, the two major sites of glycogen storage are liver and skeletal muscle. In liver, glycogen synthesis and degradation are regulated to maintain blood-glucose levels, while stored glycogen in muscle can be processed to meet the energy needs of the muscle itself. Abnormal ability to utilize glycogen is found in patients suffering diabetes and other genetic glycogen storage related diseases. BioVision EZScreenTM Glycogen Colorimetric Assay Kit is an easy and convenient assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen releasing glucose which is then specifically oxidized to produce a product that reacts with OxiRed Probe to generate color (OD 590 nm). The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The assay can detect as low as 0.025 µg of glycogen in 384-well assay format.

• Detection method: Absorbance (590 nm) • Species reactivity: Mammalian • Applications: The assay can detect glycogen as low as 0.025 µg

• Simple procedure; takes ~ 2 hours • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Glycogen

Glycogen

none

• Hydrolysis Buffer • Development Buffer • OxiRed Probe • Hydrolysis Enzyme Mix • Development Enzyme Mix • Glycogen Standard (2.0 mg/ml)

-20°C

gel pack

12 months

The assay can detect glycogen as low as 0.025 µg

• Measurement of Glycogen in various tissues/cells • Analysis of metabolism an cell sginaling in various cells

• Animal tissues: Liver, muscle etc. • Cell culture: Adherent or suspension cells.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.