EZCell™ Phagocytosis Assay Kit (Green E. coli)

Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. E. coli and other bacterial strains are often used as a pathogen in phagocytosis assays. BioVision’s EZCell™ Phagocytosis Assay Kit (Green E. coli) utilizes heat-killed, fluorescently pre-labeled E. coli particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptor (TLR) ligands.

• Detection method: Fluorescence (Ex/Em = 490/520 nm) • Applications: • Rapid detection, quantification and validation of phagocytosis

• Simple & Rapid Protocol • Convenient: Non-Radioactive, no special handling or disposal required • High-Throughput • Accurate: reproducible results with low intra & inter assay variability

Phagocytosis Assay

none

• Phagocytosis Assay Buffer • Green E. Coli • 10X Quenching Solution

+4°C

gel pack

12 months

Mammalian

• Screening for potential inhibitors that disrupt the interaction between p53 and MDM2

Phagocytic cell culture: adherent or suspension cells capable of phagocytosis

Biovision supplies other types of Assays as 1.