Mitochondrial Complex I Activity Colorimetric Assay Kit

Mitochondrial Complex I or NADH:ubiquinone oxidoreductase (EC 1.6.5.3) is the first and the largest complex of the Electron Transport Chain located in the mitochondrial membrane. It oxidizes NADH to NAD+ and transfers an electron to ubiquinone (also present in the inner mitochondrial membrane) converting it to ubiquinol. During this process, it transports protons across the inner mitochondrial membrane, helping to develop an electrochemical gradient. This process is very important for cellular respiration and adverse effects on Complex I activity can compromise mitochondrial respiration, which further leads to cellular stress. BioVision’s Mitochondrial Complex I assay kit is a fast and reliable method to determine the activity of complex I in isolated mitochondria. It is useful for respiration studies in isolated mitochondria and may be used to study effects of toxicants, drugs and other environmental conditions on mitochondrial complex I activity. This kit uses decylubiquinone, an analog of ubiquinone, as an electron acceptor that gets converted to decylubiquinol through the catalytic activity of Complex I. The Complex I dye that absorbs light at 600 nm in its oxidized form is used as a terminal electron acceptor that accepts electrons from decylubiquinol. Complex I activity is determined colorimetrically by recording the change in absorbance of reduced Complex I dye at 600 nm. Specific Complex I activity is obtained by subtracting the activity in presence of Complex I inhibitor rotenone from total activity. This kit can detect as low as 0.1 mU / well and is linear up to 7 mU / well.

• Detection method: Colorimetric (Absorbance 600 nm) • Applications: Measurement of Complex III activity in isolated mitochondria

• Simple, rapid & convenient assay to measure Mitochondrial Complex I • Includes Inhibitor (Rotenone)

Other metabolism-related assays

none

• Complex I Assay Buffer • NADH • Decylubiquinone • Complex I Dye • Complex I Inhibitor Rotenone • Clear 96-well half area plate

-20°C

gel pack

12 months

Multiple species

Measurement of Complex I activity in isolated mitochondria

Isolated mitochondria

mitochondrial

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.