PicoProbe™ D-2-Hydroxyglutarate (D2HG) Assay Kit

In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and α-Ketoglutarate. In human cancers, IDH mutations can cause a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of α-ketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is an important biomarker for early diagnosis, prognosis and the development of therapeutic strategies against these maladies. BioVision’s PicoProbe™ D-2-Hydroxyglutarate Assay Kit provides a convenient method to detect low levels of D2HG in biological samples. In this assay, D-2-Hydroxyglutarate is oxidized to α-Ketoglutarate in the presence of D2HG Enzyme Mix and Substrate Mix. The intermediate reduces the PicoProbe™ to generate fluorescence which is directly proportional to the amount of D2HG present in the samples. This assay kit is fast, sensitive, easy to use and high-through adaptable. It can measure D-2-Hydroxyglutarate levels less than 0.1 µM in various samples.

• Detection method: Fluorescence (Ex/Em 535/587 nm). • Species reactivity: Mammalian • Applications: Measurement of D2HG level in various cell/tissues/biological fluids

• Simple, fast, HTP adaptable and convenient assay • Can measure D2HG levels as low as 0.1 µM in various samples

Metabolism

none

D2HG Assay Buffer PicoProbeTM (in DMSO) D2HG Enzyme D2HG Developer D2HG Standard

-20°C

gel pack

12 months

Mammalian

Measurement of D2HG level in various cell/tissues/biological fluids.

Biological Samples: Plasma, Serum

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit