Factor XIa Activity Assay Kit (Colorimetric)

Factor XI or plasma thromboplastin antecedent (EC 3.4.21.27), the zymogen form of factor XIa, is a serine protease. In humans, Factor XI is encoded by the F11 gene. Factor XI (FXI) circulates as a homo-dimer in its inactive form activated into factor XIa by factor XIIa (FXIIa) via the "contact pathway", thrombin, and self-activation by its active form (FXIa). The deficiency of factor XI causes the rare hemophilia C which is an autosomal recessive disorder characterized by lower spontaneous bleeding, but excessive blood loss during surgical procedures. Low levels of factor XI also occur in many other disease states, including Noonan syndrome. High levels of factor XI have been implicated in thrombosis. Factor XIa also activates factor IX which, in turn, activates factor X in the coagulation cascade. BioVision's Factor XIa activity assay kit utilizes the ability of factor XIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD 405 nm). The kit is easy-to-use and can detect Factor XIa (as low as 1 mPEU) from plasma and purified protein samples.

• Detection method: Colorimetric (Absorbance 405 nm) • Applications: Measurement of FXIa activity in plasma and purified/crude enzyme preparations

• Simple, rapid & convenient assay to measure Factor XIa • Includes Positive Control and Factor Xia and Factor XIIa Inhibitors

Metabolism

none

• FXIa Assay Buffer • FXIa Activator • Inhibitor 1 • Inhibitor 2 • FXIa Substrate • Human Factor XIa • pNA Standard (0.1 M)

-20°C

gel pack

12 months

Mammalians

Measurement of Factor XIIIa activity in plasma samples and purified/crude enzyme preparations

• Plasma • Purified enzyme

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.