Nitric Oxide Cell-Based HTS Assay Kit

Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO has been established as one of the key regulators in cardiovascular, nervous, and immune systems. NO plays a pivotal role in numerous processes: in central nervous system, NO participates in cell communication and information storage; in vascular endothelium, NO is involved with regulation of vascular function. Finally, NO is produced by immune cells, including macrophages, as a part of body’s defense mechanism during immunological responses and oxidative stress conditions. However, chronic overproduction of NO is one of the fundamental causes underlying disorders including neurodegenerative diseases and pathophysiology of blood vessels. BioVision’s Nitric Oxide Cell-Based HTS Assay Kit utilizes a dye that reacts with intracellular NO to produce a highly-Fluorescent Triazole Product (Ex/Em= 488/532 nm). The kit includes Diphenyleneiodonium (DPI), a potent inhibitor of nitric oxide cellular production, which has been widely used to inhibit NO synthesis in order to evaluate NO function in different systems and diseases. This kit provides a simple, standardized and HTS method to quantitate the amount of NO in cell-based assays. This Assay Kit is user-friendly, sensitive and can detect the NO Fluorescent product as low as 5 pmol in a 96-well plate

Ultrasensitive, Simple, HTP adaptable fluoromotericassay kit for concentration of nitric oxide. Can detect as low as 5 pmol of Nitric Oxide concentration Detection method: Fluorometric analysis (Ex/Em = 488/532 nm) Applications: Detection of nitric concentration concentrations in cells Sample Type: adherent or suspension cells

Simple, HTP adaptable protocol Sensitivity as low as 5 pmol of nitric oxide in cells Fluorometric detection

Oxidative stress, Metabolism

none

NOS Assay Buffer NO Staining Dye NO Inhibitor (DPI, 1 mM) NO Standard (100 µM)

-20°C

gel pack

12 months

Mammalian cell culture

Detection of concentration of nitric oxide

Adherent or suspension cells

cell

Biovision supplies other types of Assays as 1.

For cells, cell lines and tissues in culture till half confluency.