5-Lipoxygenase Inhibitor Screening Kit (Fluorometric)

5-Lipoxygenase (5-LOX) is a non-heme iron-containing dioxygenase that converts unsaturated fatty acids to epoxides; for example the synthesis of leukotrienes from arachidonic acid. It is involved in processes like cell proliferation, differentiation and inflammation and has been implicated in inflammation and hyper proliferation mediated diseases like asthma, rheumatoid arthritis and cancer. Research for identifying potential remedies for such diseases involves the identification of inhibitors of 5-lipoxygenase. BioVision’s 5-Lipoxygenase Inhibitor Screening Kit uses Zileuton, a well-known 5-lipoxygenase inhibitor as positive control, and provides a simple and fast method to screen/ characterize potential 5-lipoxygenase inhibitors

• Detection method: Fluorescence (Ex/Em 500/536 nm) • Species reactivity: Human • Applications :Screening of potential inhibitors of 5:lipoxygenase

• Simple procedure; takes only ~40 min • Fast and convenient

Fatty Acid Metabolism

none

• LOX Assay Buffer • LOX Probe • LOX Substrate • Zileuton • 5-LOX Enzyme

-20°C

gel pack

12 months

Human

Screening of potential inhibitors of 5-lipoxygenase

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.