3D Cell Culture Non-Enzymatic Cell Harvesting Kit

Catalog number
K982-100
Name
3D Cell Culture Non-Enzymatic Cell Harvesting Kit
Size
100 samples
Price
297.00 EUR
Supplier

Details

Kit's description
Three dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fashion. 3D cell cultures improve the function, differentiation and viability of cells and recapitulate in vivo microenvironment compared to conventional 2D cell cultures. 3D matrices provide a physiologically relevant screening platform, by mimicking the in vivo responses, for many cell types including cancer and stem cells in developmental morphogenesis, pharmacology, drug metabolism and drug toxicity studies. However, when it comes to cell passaging, or studying biochemical processes and protein analysis, there are challenges with separating intact cells from extra-cellular proteins comprising the 3D matrices. While proteases (such as trypsin and accutase) are commonly used to degrade these matrices, it is not recommended in some cases where cells are sensitive to protease digestion; these proteases may modify cellular proteins and signaling or cell surface, thus altering physiological assessments. Further, some proteases-based dissociation methods do not completely dissolve the matrices. BioVision’s 3D Culture Non-Enzymatic Cell Harvesting Kit provides an optimized and standardized saline-based solution for the isolation of cells and spheroids from matrices (especially for BioVision’s 3D Cell Culture Kits: K517, 518, 519) with high viability rate for subsequent biochemical, protein and cell-based analysis.
Highlights
• Detection method: Qualitative • Applications: Matrix and cell/spheroid dissociations • Recommended for experiment where cells are sensitive to protease digestion
Kit's benefits
• Easy, Fast, Reproducible, Qualitative tool for screening, studying, and characterizing compounds in cell culture studies • Recommended for experiment where cells are sensitive to protease digestion
Category
Cell based assays, 3D Cell Culture
Kit's other name
none
Contents
• Matrix Dissociation Saline Solution • Neutralization
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Recommended for experiment where cells are sensitive to protease digestion
Applications
Matrix and Cell/Spheroid DissociationsCell-harvesting for usage in cell-based assay, biochemical, and protein analysisRecommended for experiment where cells are sensitive to protease digestion.
Samples tested
3D Matrices• Adherent and suspension spheroids and cells
Tissue
cell
Description
For cells, cell lines and tissues in culture till half confluency.