Senescence Detection Kit (Fluorometric)

Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of β-galactosidase (SA-β-Gal) activity, and altered patterns of gene expression. The Senescence detection kit is designed to fluorescently detect SA-β-Gal activity in cultured cells by FACS. The SA-β-Gal is present only in senescent cells and is not found in pre-senescent, quiescent or immortal cells

• Detection method: FACS (FL1 channel) • Application:Detection of senescence cells :Screening potential senescence inducers/inhibitors

Simple, one step protocol

Senescence

none

• Wash Buffer • Senescence Dye

-20°C

gel pack

12 months

Mammalian

Detection of senescence cells

Adherent/Suspension Cells

The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.