Factor XIIa Activity Assay Kit (Colorimetric)

Factor XII or Hageman factor (EC 3.4.21.38), is the zymogen form of factor XIIa, a serine protease involved in Coagulation Pathway. This single chain zymogen is activated by Kallikrein into a two-chain serine protease (XIIa) with a heavy chain (factor XIIa-alpha) and a light chain. Human Factor XII and Prekallikrein are thought to be involved in a reciprocal activation mechanism in which Factor XIIa activates Prekallikrein to Kallikrein, which in turn converts Factor XII to XIIa. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. The defects in this gene do not cause any clinical symptoms but prolong the whole blood clotting time. BioVision's Factor XIIa activity assay kit utilizes the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm). The kit is easy-to-use and can detect Factor XIIa (as low as 1 mPEU) from plasma sampless.

• Detection method: Colorimetric (Absorbance 405 nm) • Applications: Measurement of FXIIa activity in plasma and purified/crude enzyme preparations

• Simple, rapid & convenient assay to measure Factor XIa • Includes Positive Control and Factor XIIa and Factor XIIa Inhibitors

Metabolism

none

• FXIIa Assay Buffer • FXIIa Activator • FXIIa Substrate • Human Factor XIIa • Human Factor XIIa Supplement • FXIIa Inhibitor • pNA Standard (0.1 M)

-20°C

gel pack

12 months

Mammalians

Measurement of Factor XIIa activity in plasma samples and purified/crude enzyme preparations

Plasma and Purified enzyme

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.