TNF alpha (rat) ELISA Kit

BioVision’s rat TNF alpha ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for rat TNF alpha coated on a 96-well plate. Standards and test samples are added to the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for TNF alpha is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat TNF alpha captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml (cell culture supernates), 7.8 pg/ml – 500 pg/ml (rat serum, plasma). Sensitivity: < 1 pg/ml.

• Detection method: Absorbance (450 nm) • Species reactivity: rat • Application: Quantitative protein detection, establishing normal range etc.

• Easy, convenient and time-saving method to assay for rat TNF alpha. • Detection Range:15.6 pg/ml – 1000 pg/ml (cell culture supernates), 7.8 pg/ml – 500 pg/ml (rat serum, plasma). Sensitivity: < 1 pg/ml. • This ELISA kit shows no cross-reactivity with other relevant proteins.

TNF alpha

Tumor Necrosis Factor alpha (rat) ELISA Kit

• TNF alpha Microplate coated with anti-rat monoclonal Ab against TNF alpha, 96 wells • Lyophilized recombinant rat TNF alpha standard (10 ng/vial) • Biotinylated anti-rat TNF alpha antibody • Avidin-Biotin-Peroxidase Complex (ABC) • Sample diluent buffer • Antibody diluent buffer • ABC diluent buffer • TMB color developing agent (Colorless) • TMB stop solution

-20°C

gel pack

12 months

rat

Application- Quantitative protein detection, establishing normal range.

• Serum & plasma (heparin, EDTA) • Cell culture supernatants

The TNF alpha ( ) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Rattus norvegicus

Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.