β-catenin (human) ELISA Kit

β-catenin (cadherin-associated protein beta, 88 kDa) is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription via the Wnt signaling pathway. Mutations and overexpression of β-catenin are associated with many cancers, including hepatocellular, colorectal, lung, breast, ovarian and endometrial cancers. BioVision’s human β-catenin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human β-catenin captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 0.156 – 10 ng/ml.

• Detection method: Absorbance (450 nm) • Species reactivity:human • Application: Quantitative protein detection, establishing normal range etc.

• Easy, convenient and time-saving method to assay for human β-catenin. • Detection Range: 0.156 – 10 ng/ml. • This ELISA kit shows no species cross-reactivity.

β-catenin ELISA kit

β-catenin (human) ELISA kit

• 96 wells coated with anti-human β-catenin antibody, 1 Microplate • Human β-catenin standard (18 ng/ml) • HRP-conjugate reagent • Standard Diluent • Sample Diluent • Chromogen Solution A • Chromogen Solution B • Stop Solution • Wash Solution (30x stock) • Sealed Bag • Plate cover

4°C

gel pack

12 months

human

Application- Quantitative detection of β-catenin, establishing normal range.

• Serum and other biological fluids • Cell culture medium, tissue homogenates and cell lysates

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED