β-catenin (mouse) ELISA Kit

β-catenin (cadherin-associated protein beta, 88 kDa) is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription via the Wnt signaling pathway. Mutations and overexpression of β-catenin are associated with many cancers, including hepatocellular, colorectal, lung, breast, ovarian and endometrial cancers. BioVision’s mouse β-catenin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Mouse β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse β-catenin captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 3 – 180 ng/L.

• Detection method: Absorbance (450 nm) • Species reactivity:mouse • Application: Quantitative protein detection, establishing normal range etc.

• Easy, convenient and time-saving method to assay for mouse β-catenin. • Detection Range: 3 – 180 ng/L. • This ELISA kit shows no species cross-reactivity.

β-catenin ELISA kit

β-catenin (mouse) ELISA kit

• 96 wells coated with anti-mouse β-catenin antibody, 1 Microplate with 2 adhesive strips • Mouse β-catenin standard (270 ng/L) • HRP-conjugate reagent • Standard Diluent • Sample diluent • Chromogen Solution A • Chromogen Solution B • Stop Solution • Wash Solution (30x stock)

4°C

gel pack

12 months

mouse

Application- Quantitative detection of β-catenin, establishing normal range.

• Serum & plasma (EDTA/Citrate), urine and other biological fluids • Cell culture medium, tissue homogenates and cell lysates

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus