β-catenin (rat) ELISA Kit

β-catenin (cadherin-associated protein beta, 88 kDa) is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription via the Wnt signaling pathway. Mutations and overexpression of β-catenin are associated with many cancers, including hepatocellular, colorectal, lung, breast, ovarian and endometrial cancers. BioVision’s rat β-catenin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Rat β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat β-catenin captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 5 – 240 ng/L.

• Detection method: Absorbance (450 nm) • Species reactivity:rat • Application: Quantitative protein detection, establishing normal range etc.

• Easy, convenient and time-saving method to assay for rat β-catenin. • Detection Range: 5 – 240 ng/L. • This ELISA kit shows no species cross-reactivity.

β-catenin ELISA kit

β-catenin (rat) ELISA kit

• 96 wells coated with anti-rat β-catenin antibody, 1 Microplate with 2 adhesive strips • Rat β-catenin standard (360 ng/L) • HRP-conjugate reagent • Standard Diluent • Sample diluent • Chromogen Solution A • Chromogen Solution B • Stop Solution • Wash Solution (30x stock)

4°C

gel pack

12 months

rat

Application- Quantitative detection of β-catenin, establishing normal range.

• Serum & plasma (EDTA/Citrate), urine and other biological fluids • Cell culture medium, tissue homogenates and cell lysates

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Rattus norvegicus