Fibronectin (human) ELISA Kit

Fibronectin is a 430 KDa dimeric glycoprotein that exists in 2 forms - cellular and plasma fibronectin. BioVision’s human Fibronectin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rabbit polyclonal antibody specific for human Fibronectin is coated on a 96-well plate. Standards and test samples are added to the wells and Fibronectin present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Fibronectin is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Fibronectin captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 10 pg/ml.

• Detection method: Absorbance (450 nm) • Species reactivity:human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

• Easy, convenient and time-saving method to assay for human Fibronectin. • Detection Range:156 pg/ml – 10,000 pg/ml. Sensitivity: < 10 pg/ml. • This ELISA kit shows no cross-reactivity with other relevant proteins.

Fibronectin ELISA kit

Fibronectin (human) ELISA Kit

• 96 wells coated with anti-human polyclonal Fibronectin antibody, 1 Microplate • Lyophilized native human Fibronectin standard (10 ng/vial) • Biotinylated anti- human Fibronectin antibody • Avidin-Biotin-Peroxidase Complex (ABC) • Sample diluent buffer • Antibody diluent buffer • ABC diluent buffer • TMB color developing agent (Colorless) • TMB stop solution

-20°C

gel pack

12 months

human

Application- Quantitative protein detection, establishing normal range

• Serum & plasma (heparin, EDTA, Citrate) • Cell culture supernatants

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED